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Imaging a cellule vive time-lapse in vitro per esplorare la migrazione cellulare verso l'organo di Corti

Article DOI: 10.3791/61947-v • 08:08 min • December 4th, 2020
December 4th, 2020 •
*1,2,3, *1,2, 1,2, 1,2, 3
1Department of Otorhinolaryngology, Yonsei University Wonju College of Medicine, 2Research Institute of Hearing Enhancement, Yonsei University Wonju College of Medicine, 3Department of Otorhinolaryngology Head and Neck Surgery, Hallym University College of Medicine
* These authors contributed equally

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In questo studio, presentiamo un metodo di imaging in tempo reale utilizzando la microscopia confocale per osservare le cellule che si muovono verso il tessuto danneggiato mediante incubazione ex vivo con l'epitelio cocleare contenente l'organo di Corti.

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Questo mese a JoVE Numero 166 Organo di Corti microscopio confocale time-lapse cellule staminali mesenchimali migrazione cellule ciliate cellule di supporto immunoetichettatura sistema di cocultura
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