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5-Ethynyl-2'-Deoxyuridin/Phospho-Histone H3 Dual-Labeling Protocol för cellcykelprogressionsanalys i Drosophila neurala stamceller

Article DOI: 10.3791/62642-v • 09:34 min • May 4th, 2021
May 4th, 2021 •
1,2, 1
1Cell Biology, University of Bern, 2Department of life sciences and medicine, University of Luxembourg

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Cellcykel analys med 5-ethynyl-2'-deoxyuridin (EdU) och phospho-histon H3 (pH3) märkning är en flera steg förfarande som kan kräva omfattande optimering. Här presenterar vi ett detaljerat protokoll som beskriver alla steg för denna procedur inklusive bildanalys och kvantifiering för att skilja celler i olika cellcykelfaser.

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Biologi nummer 171
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