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Saggi di immunocitochimica delle cellule 3D del glioma diffuso della linea mediana pediatrica

Article DOI: 10.3791/63091-v • 09:06 min • November 11th, 2021
November 11th, 2021 •
*1, *1, 2,3, 1
1Department of Onco-hematology, Gene and Cell Therapy, Bambino Gesù Children's Hospital, IRCCS, 2Oncology Service, Children's Health Queensland Hospital and Health Service, 3Child Health Research Centre, The University of Queensland
* These authors contributed equally

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Questo studio presenta un protocollo di immunocitochimica delle cellule 3D vive applicato ad una linea cellulare di glioma diffuso pediatrico della linea mediana, utile per studiare in tempo reale l'espressione delle proteine sulla membrana plasmatica durante processi dinamici come l'invasione e la migrazione delle cellule 3D.

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