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Bioengineering

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Hurtig indkapsling af rekonstitueret cytoskelet inde i gigantiske unilamellare vesikler

Article DOI: 10.3791/63332-v • 07:48 min • November 10th, 2021
November 10th, 2021 •
*1, *1, 2, 3, 1,4,5,6
1Department of Mechanical Engineering, University of Michigan, Ann Arbor, 2John A. Paulson School of Engineering and Applied Sciences, Harvard University, 3Department of Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, 4Department of Biomedical Engineering, University of Michigan, Ann Arbor, 5Department of Biophysics, University of Michigan, Ann Arbor, 6Cellular and Molecular Biology Program, University of Michigan, Ann Arbor
* These authors contributed equally

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Denne artikel introducerer en simpel metode til hurtig produktion af gigantiske unilmellære vesikler med indkapslede cytoskeletale proteiner. Metoden viser sig at være nyttig til bottom-up rekonstitution af cytoskeletale strukturer i indespærring og cytoskelet-membraninteraktioner.

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Bioengineering Udgave 177 Emulsionsoverførsel cDICE GUV'er bottom-up rekonstitution actin fascin
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