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Rask innkapsling av rekonstituert cytoskjelett inne i gigantiske unilamellar vesicles

Article DOI: 10.3791/63332-v • 07:48 min • November 10th, 2021
November 10th, 2021 •
*1, *1, 2, 3, 1,4,5,6
1Department of Mechanical Engineering, University of Michigan, Ann Arbor, 2John A. Paulson School of Engineering and Applied Sciences, Harvard University, 3Department of Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, 4Department of Biomedical Engineering, University of Michigan, Ann Arbor, 5Department of Biophysics, University of Michigan, Ann Arbor, 6Cellular and Molecular Biology Program, University of Michigan, Ann Arbor
* These authors contributed equally

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Denne artikkelen introduserer en enkel metode for rask produksjon av gigantiske unilamellar vesikler med innkapslede cytoskeletale proteiner. Metoden viser seg å være nyttig for nedenfra-og-opp-rekonstituering av cytoskeletale strukturer i innesperring og cytoskjelettmembraninteraksjoner.

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Bioengineering Utgave 177 Emulsjonsoverføring cDICE GUVer nedenfra-og-opp-rekonstituering aktin fascin
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