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JoVE Journal Biochemistry
Concanavalin A-baseret sedimentationsassay til måling af substratbinding af glucanphosphataser
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Concanavalin A-baseret sedimentationsassay til måling af substratbinding af glucanphosphataser

Article DOI: 10.3791/64700-v • 09:07 min • December 23rd, 2022
December 23rd, 2022 •
*1, *1, 1, 1, 1
1Department of Chemistry, Skidmore College
* These authors contributed equally

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Denne metode beskriver et lektinbaseret in vitro-sedimentationsassay til kvantificering af bindingsaffiniteten af glucanphosphatase og amylopectin. Dette co-sedimenteringsassay er pålideligt til måling af glucanphosphatasesubstratbinding og kan påføres forskellige opløselige glucansubstrater.

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Tilbagetrækning nummer 190
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