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JoVE Journal Biochemistry
Concanavalin A-baserad sedimentationsanalys för att mäta substratbindning av glukanfosfataser
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Concanavalin A-baserad sedimentationsanalys för att mäta substratbindning av glukanfosfataser

Article DOI: 10.3791/64700-v • 09:07 min • December 23rd, 2022
December 23rd, 2022 •
*1, *1, 1, 1, 1
1Department of Chemistry, Skidmore College
* These authors contributed equally

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Denna metod beskriver en lektinbaserad sedimentationsanalys in vitro för att kvantifiera bindningsaffiniteten för glukanfosfatas och amylopektin. Denna samsedimenteringsanalys är tillförlitlig för mätning av glukanfosfatassubstratbindning och kan appliceras på olika lösliga glukansubstrat.

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