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HIGH SCHOOL

Chemistry

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Chemistry

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Analytical Chemistry

Ion-Exchange Chromatography for Sample Cleanup

Description

Ion-exchange chromatography is a separation method that cleans up samples by using charge. A column is packed with an ion-exchange resin, which is a charged stationary phase on a solid support. Strong cation-exchange chromatography uses a negatively charged resin to separate cations. Strong anion-exchange chromatography uses...

Transcript

La chromatographie par échange d’ions est largement utilisée dans la séparation et l’isolement des composés chargés, en particulier les grandes biomolécules.

Ce type de chromatographie liquide utilise une colonne de billes de phase stationnaire emballées, appelée résine. La technique sépare les analytes en fonction de leur affinité avec l...

Tags

Cation Exchange ChromatographyAnion Exchange ChromatographyProtein SeparationSalt Gradient ElutionColumn EquilibrationBuffer pH SelectionHydroxyapatite ChromatographyImmobilized Metal Affinity ChromatographyHigh Performance Liquid Chromatography

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