Name: centrosome duplication in cell division
Uploaded: 2023-04-30T18:28:50.000Z
Duration: 2 min 25 s
Channel: JoVE
Description: Centrosome duplication is an important step in cell division. The centrosome helps organize the cell’s internal structure, and it must be copied before a cell divides.

K12 Standards

Pennsylvania State Academic Standards

9-12

Life Science

Inheritance and Variation of Traits

3.1.9-12.D

Centrosome Duplication

Un centrosome est le principal organisateur de microtubules d’une cellule eucaryote et joue un rôle essentiel dans la formation du fuseau mitotique, un arrangement bipolaire de microtubules qui sépare les chromatides sœurs. Les deux pôles du fuseau sont formés par deux centrosomes placés de manière opposée.
Avant le cycle cellulaire, cependant, la plupart des cellules animales ne contiennent qu’un seul centrosome dans le cytoplasme. La kinase dépendante G1/S-cycline ou complexe Cdk, composée de la cycline E et de la Cdk2 dans les cellules animales, aide à déclencher la duplication des centrosomes ainsi que l’entrée dans le cycle cellulaire. 
Chaque centrosome est constitué de deux centrioles, qui sont encapsulés par une matrice péricentriolaire protéique.
La duplication des centrosomes commence au début de la phase G1 lorsque la séparatrice effectue le désengagement des deux centrioles. Une protéine appartenant à la famille des protéines kinases de type polo, Plk-4, est un régulateur important de la biogenèse des centrioles chez l’homme.
Vers le début de la phase S, un centriole fille commence à se développer à angle droit par rapport à la base de chaque centriole.
Au cours de G2, les centrioles filles finissent généralement de s’allonger. Les quatre centrioles restent rapprochés dans le centrosome jusqu’à ce que la cellule entre en mitose.
Au début de la mitose, le centrosome se divise en deux, ce qui est également connu sous le nom de disjonction du centrosome. Les deux centrosomes résultants contiennent chacun une paire de centrioles et la matrice péricentriolaire. Les deux centrosomes nucléent un ensemble de microtubules, principalement au centriole mère, formant collectivement le fuseau mitotique.
Le cycle de vie des centrosomes et des chromosomes est similaire à plusieurs égards. Leur duplication ne se produit qu’une fois par cycle et les deux sont dupliqués par un processus semi-conservateur où une nouvelle copie est produite à partir de la copie parent préexistante.
De plus, comme pour les chromosomes, une copie du centrosome est distribuée de manière égale à chaque cellule fille après la division.
Une duplication excessive des centrosomes peut altérer l’assemblage du fuseau mitotique et provoquer une mauvaise ségrégation des chromosomes, ce qui peut provoquer des maladies comme le cancer.

<ol>
	<li style="margin-left : 0.5in ;">Fujita, Hiroki, Yuki Yoshino et Natsuko Chiba. 2015. « Régulation du cycle des centrosomes. » Oncologie moléculaire et cellulaire 3 (2). https://doi.org/10.1080/23723556.2015.1075643.</li>
	<li style="margin-left : 0.5in ;">Nigg, Erich A., et Andrew J. Holland. 2018. « Une fois et une seule fois : mécanismes de duplication des centrioles et leur dérégulation dans la maladie. » Nature Reviews Molecular Cell Biology 19 (5) : 297-312. https://doi.org/10.1038/nrm.2017.127.</li>
</ol>

centrosome duplication

Le principal centre organisateur des microtubules (MTOC) dans les cellules animales est le centrosome. Un centrosome possède en son cœur deux centrioles cylindriques. Chaque centriole est constitué de neuf ensembles de trois microtubules maintenus ensemble par des protéines. Les centrioles sont positionnés à angle droit les uns par rapport aux autres et entourés d'un nuage de protéines informe appelé matrice péricentriolaire, ou matériau péricentriolaire (PCM).

Pour garantir que chaque cellule fille reçoive un centrosome après la division cellulaire, la duplication du centrosome commence tôt dans le cycle cellulaire. La duplication des centrosomes est étroitement régulée par les contrôles du cycle cellulaire- tels que la kinase 2 dépendante de la cycline (Cdk2)- pour éviter qu'elle ne se produise plus d'une fois par cycle cellulaire. Ainsi, au moment où la cellule atteint la mitose, elle possède deux centrosomes.

La duplication des centrosomes coïncide avec les phases du cycle cellulaire. Au cours de la phase G_1 du cycle cellulaire, les deux centrioles du centrosome se séparent, un processus appelé désorientation du centrosome.

Durant les phases G1 et S, les centrosomes sont dupliqués. Un nouveau centriole, appelé procentriole, commence à se former et à s'allonger à la base de chacun des deux centrioles existants. Les procentrioles s'allongent jusqu'à S et G2 jusqu'à ce qu'ils soient aussi grands que les centrioles plus âgés. Les quatre centrioles restent rapprochés dans le PCM élargi jusqu'à ce que la cellule entre en mitose.

Au cours de la phase G2, la γ-tubuline et d'autres protéines PCM s'accumulent dans le centrosome, un processus appelé maturation du centrosome.

Lors de la transition entre les phases G2 et M, les centrosomes commencent à se séparer. Les deux centrioles mères se déconnectent et les protéines motrices des microtubules séparent les deux centrosomes.

Des erreurs dans la régulation des centrosomes peuvent entraîner des anomalies dans le nombre de chromosomes et de centrosomes. Les anomalies des centrosomes et les défauts de progression du cycle des centrosomes sont impliqués dans plusieurs maladies, notamment le cancer. Les protéines suppressives de tumeurs et les oncogènes sont liés à des modifications néfastes des centrosomes des cellules tumorales, ce qui fait de ces protéines une cible thérapeutique attrayante.

Le principal centre d’organisation des microtubules (MTOC) dans les cellules animales est le centrosome. Un centrosome a deux centrioles cylindriques en son cœur. Chaque...

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centrosome duplication in cell division

Centrosome duplication is an important step in cell division. The centrosome helps organize the cell’s internal structure, and it must be copied before a cell divides.

Centrosome Duplication in Cell Division

mitosis and cytokinesis

Stages of Cell Division in Eukaryotes

Cell division in eukaryotes follows a tightly regulated cycle that includes cell growth, DNA replication, chromosome duplication, chromosome distribution to daughter cells, and cell division. Regulatory systems and extracellular signals help control when cells divide. In typical human cells, the cycle takes about 24 hours. The cycle has two major stages. DNA is copied during the S phase of interphase. The mitotic, or M, phase then separates the duplicated chromosomes into two new nuclei and...

chromosome duplication

Chromosome Copying in Cell Division

Chromosome copying is an important step in cell division. Before a cell divides, it must make an exact copy of its chromosomes so each new cell can receive the same genetic information. A chromosome is a DNA-containing structure that carries genes. During duplication, the chromosome is copied into two identical sister chromatids. These copies stay attached until the cell is ready to separate them. This process helps ensure that daughter cells inherit a complete set of chromosomes. Accurate...

cohesins

Cohesins in Sister Chromatid Separation

Cohesins are protein complexes that help hold sister chromatids together after DNA is copied. They play a key role in cell division by keeping the two copies of a chromosome connected until the right time. These proteins act like a link between the sister chromatids. When the cell is ready to separate them, cohesins must be removed or released so each new cell can get one complete set of chromosomes. Cohesins are an important part of chromosome behavior during mitosis and meiosis. Their...

condensins

Condensin Proteins in Chromosome Packing

Condensin proteins help pack DNA into condensed chromosomes during cell division. They are part of the larger set of proteins that organize genetic material so it can be separated correctly when a cell divides. These proteins act by shaping and tightening chromosome structure. This process is important because DNA must be compacted before it can move through the stages of division. Condensins are therefore closely linked to chromosome condensation, the step that makes chromosomes more compact...

the mitotic spindle

How the Mitotic Spindle Guides Cell Division

The mitotic spindle guides chromosome movement during cell division. It is a structure made of microtubules, which are thin protein fibers in the cell. These fibers organize and help separate the genetic material before the cell splits. The spindle forms as the cell prepares to divide. Its fibers attach to chromosomes and line them up so they can be moved apart in an orderly way. This careful organization helps each new cell receive the correct set of chromosomes. The mitotic spindle is a key...

Centrosome duplication is an important step in cell division. The centrosome helps organize the cell’s internal structure, and it must be copied before a cell divides.

spindle assembly

Spindle Formation in Cell Division

Spindle formation in cell division is the process that builds the microtubule-based spindle needed to separate chromosomes. Microtubules are long protein fibers that help move and organize cell parts during division. During this process, microtubules grow and are arranged into a spindle structure around the chromosomes. The spindle forms in a way that supports chromosome movement to opposite sides of the cell. This step is essential for accurate cell division. Spindle formation depends on the...

attachment of sister chromatids

Sister Chromatid Attachment in Mitosis

Sister chromatid attachment in mitosis helps align duplicated chromosomes before they separate. Sister chromatids are the matching copies made after DNA replication. They stay connected until the cell is ready to divide. The attachment step is closely tied to the metaphase plate, the middle of the cell where chromosomes line up. This alignment helps each new cell receive one copy of every chromosome. Proper attachment is an important part of accurate cell division.

forces acting on chromosomes

Chromosome Movement During Cell Division

Chromosome movement during cell division depends on forces that act on the chromosomes. These forces help move the chromosomes into the right position as the cell divides. The transcript focuses on how these forces affect chromosome behavior during division. It highlights the role of the mitotic spindle, which is the structure that helps organize and move chromosomes. The spindle uses force to guide chromosome movement at the right time. Understanding these forces helps explain how...

separation of sister chromatids

Chromatid Pulling in Cell Division

Separation of sister chromatids is a key step in cell division. Sister chromatids are two identical copies of a chromosome that stay attached until the cell is ready to split them apart. Their separation helps each new cell receive the correct genetic material. During this stage, the sister chromatids are pulled to opposite sides of the cell. This movement happens as the cell prepares to divide into two daughter cells. Once the chromatids separate, each side of the cell has a complete set of...

the spindle assembly checkpoint

How Cells Verify Chromosome Spindle Attachment

The spindle assembly checkpoint helps cells verify that chromosomes are attached to the spindle before division continues. It acts as a safety step during cell division and helps prevent errors in chromosome separation. This checkpoint is controlled by kinetochores, which are protein structures on chromosomes that connect to spindle fibers. If a chromosome is not attached correctly, the checkpoint can delay progression until the problem is fixed. Once the attachment is correct, the cell can...

anaphase a and b

Chromosome and Spindle Movement in Anaphase

Anaphase is the stage of cell division when chromosomes move apart. In this phase, sister chromatids separate and travel toward opposite poles of the cell. Chromosome movement in anaphase can happen in two ways. In anaphase A, chromosomes move closer to the poles as the microtubules attached to them shorten. In anaphase B, the spindle poles themselves move farther apart and help stretch the cell. These two movements work together to ensure that the separated chromosomes are pulled to opposite...

anaphase promoting complex

Cell Cycle Control by APC

The anaphase-promoting complex, or APC, helps control cell cycle progression. It acts during cell division to help move the process from one stage to the next. This control is important for proper timing in the cell cycle. APC is a protein complex that works as a regulator. It helps trigger key steps that allow division to continue in an ordered way. By guiding this transition, APC supports normal cell cycle control.

the contractile ring

Cell Division and the Contractile Ring

The contractile ring helps cells divide at the end of cell division. It forms at the cell's center and tightens to split one cell into two. This process is part of cytokinesis, the stage when the cytoplasm divides. The ring is made of actin and myosin, two proteins that can work together to create force. As the ring contracts, it draws the cell membrane inward. This pinch helps separate the two daughter cells. Understanding the contractile ring is important for studying how cells complete...

determining the plane of cell division

Spindle Position and Cell Division Plane

The plane of cell division is set by the position of the spindle during mitosis. Cells use this internal structure to help decide where the cell will split. The result affects how the two daughter cells are arranged after division. This process is important in many developing tissues. It helps explain how cells divide in a controlled direction. A clear plane of division supports proper cell organization as new cells form. The transcript focuses on how the spindle is used to determine that...

the phragmoplast

Phragmoplast in Plant Cell Division

The phragmoplast is a plant cell structure that forms during cell division. It helps guide the final stage of division, when the cell begins to split into two daughter cells. In plant cells, the phragmoplast appears between the two new nuclei. It is part of the process that leads to the formation of a new cell wall. This wall separates the daughter cells after division is complete. The phragmoplast plays an important role in plant cytokinesis, which is the physical division of the cytoplasm.

distribution of cytoplasmic content

Cytoplasmic Content During Cell Division

Cytoplasmic content is distributed as a cell divides. This process helps each new cell receive the materials it needs to function. The cytoplasm includes the fluid and structures inside the cell membrane, but outside the nucleus. During cell division, this content is separated between the two daughter cells. The division must be organized so both cells can continue normal activity after the split. The video focuses on how this distribution occurs as part of cell division. It highlights the...

key terms and concepts

Learn how centrosome duplication supports cell division and helps a cell prepare for the next stage of growth and division in grades 9–12 biology.

Centrosome Duplication in Cell Division | JoVE K12

cell biology

JoVE Core Biologie cellulaire illustre les processus cellulaires complexes dans des leçons vidéos complètes mais faciles à comprendre. Cette collection se concentre sur les composants et fonctions essentiels de la cellule ; cela inclut les organelles et les membranes ainsi que le transport et le métabolisme énergétique cellulaire. Elle est complétée par nos vidéos de scientifiques à l'action qui démontrent l'application de la biologie cellulaire dans des expériences concrètes.

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3.1.9-12.P

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3.1.9-12.Q

Centrosome Duplication in Cell Division

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