Generating Murine Model of Myocardial Infarction: A Surgical Procedure for Permanent Ligation of Left Anterior Descending Coronary Artery in Mouse Model

Abstract

Source: Lugrin, J. et al. Murine Myocardial Infarction Model using Permanent Ligation of Left Anterior Descending Coronary Artery. J. Vis. Exp. (2019)

In this video, we describe a surgical procedure to generate a mouse model of myocardial infarction by permanently ligating the left anterior descending coronary artery.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Anesthesia and Tracheal Cannulation

1. Weigh the mouse to determine the dosage of anesthetic drugs, post-operative analgesic medication and tidal volume of the ventilator. Pre-warm the heating pad at 37 °C. The surgical setup is depicted in Figure 1.
2. Inject mouse intraperitoneally with a mix of ketamine and xylazine at a dose of 80 mg/kg and 10 mg/kg, respectively.
3. Quickly shave the mouse fur on the throat and the left side of t....

Results

Figure 1: Description of the surgical setup. (A) Surgical setup comprises a modified heating pad, a ventilator and retractors attached to elastic bands. (B) Set of scissors, forceps and need.......

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
30G Needle	BD Microlance 3	304000
Blunt Retractors	Fine Science Tools	18200-09
Cotton Swabs	Applimed SA	6001109
Dissecting Scissors, Curved	Aesculap	BC603R
Electrical Razor	Remington	HC720
Hair Removal Cream, Veet	Silk & Fresh Tech.	8218535
Micro Scissors, Curved Blunt/Blunt	Aesculap	FM013R
1 CC Syringe, Omnifix-F	B. Braun	9161406V
Silk Suture 5-0, BB	Ethicon	K880H
Silk Suture 6-0, P-1	Ethicon	639H
Silk Suture 7-0, BV-1	Ethicon	K804
Student Dumont #7 Forceps	Fine Science Tools	91197-00
Student Fine Forceps, Angled	Fine Science Tools	91110-10
Surgical Gloves	Weitacare	834301
Surgical heating pad	Personalized setting
Temgesic sol 0.3 mg/mL Buprenorphine	Indivior Schweiz AG	N02AE01
Tracheal tube inner needle of an 16G i.v. cat	Abbocath-T	G714-A01
Universal S3 Microscope, OMPIMD	Zeizz
Ventilator, MiniVent Model 845	Harvard Apparatus	73-0043
Viscotears	Alcon	1551535
70% Ethanol
Betadine 60 mL	MundiPharma