Pancreatic Intravital Imaging Window Installation in Mouse Model: An Integrated Pancreatic Imaging Window for Time-lapse In Vivo Imaging of Murine Pancreas Microstructure

Abstract

Source: Park, I. et. al. Stabilized Longitudinal In Vivo Cellular-Level Visualization of the Pancreas in a Murine Model with a Pancreatic Intravital Imaging Window. J. Vis. Exp. (2021)

This video demonstrates the procedure to implant a pancreatic imaging window to separate the pancreas from the intestines for longitudinal time-lapse high-resolution imaging of the pancreas microstructure.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Surgery

1. Prepare a sterile surgical platform and sterilize the surfaces with 70% ethanol.|
   NOTE: For longitudinal imaging sessions, aseptic technique is essential.
2. Anesthetize mice with a mixture of tiletamine/zolazepam (30 mg/kg) and xylazine (10 mg/kg).
   NOTE: Use of tiletamine/zolazepam is recommended instead of ketamine because of its adverse effect of hyperglycemia. Optimal anesthesia should be selected for the purpose of t....

Results

Figure 1. Design and photograph of the pancreatic intravital imaging window. (A) A 3D and cross-sectional view of the pancreatic intravital imaging window. (B) Anterior and posterior photograph of the pancreatic imag.......

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
Cover glasses circular	Marienfeld	111520	Cover glass for pancreatic imaging window
IRIS Scissor	JEUNGDO BIO & PLANT CO, LTD	S-1107-10	This product can be replaced with the product from other company
Loctite 401	Henkel	401	N-butyl cyanoacrylate glue
Micro Needle holder	JEUNGDO BIO & PLANT CO, LTD	H-1126-10	This product can be replaced with the product from other company
Micro rectractor	JEUNGDO BIO & PLANT CO, LTD	17004-03	This product can be replaced with the product from other company
Microforceps	JEUNGDO BIO & PLANT CO, LTD	F-1034	This product can be replaced with the product from other company
Nylon 4-0	AILEE	NB434	Non-Absorbable Suture
Omnican N 100 30G	B BRAUN	FT9172220S	For Vascular Catheter, Use only Needle part
Pancreatic imaging window	Geumto Engineering	Custom order	Pancreatic imaging window - custom order
Physiosuite	Kent Scientific	PS-02	Homeothermic temperature controller
Ring Forceps	JEUNGDO BIO & PLANT CO, LTD	F-1090-3	This product can be replaced with the product from other company
Rompun	Bayer	Rompun	Anesthetic agent
Zoletil	Virbac	Zoletil 100	Anesthetic agent
BALB/C Nude 	OrientBio 	BALB/C Nude	BALB/C Nude