Erratum: Epithelial Cell Repopulation and Preparation of Rodent Extracellular Matrix Scaffolds for Renal Tissue Development

Résumé

An erratum was issued for Epithelial Cell Repopulation and Preparation of Rodent Extracellular Matrix Scaffolds for Renal Tissue Development.

The human RCTEC/RCTE cell stocks used here and in Caralt et al., 2015, Uzarski et al., 2015 were originally provided by Dr. Loghman-Adham (then at St. Louis University) to co-author Dr. Wandinger-Ness (University of New Mexico) through an MTA in 2001. The SV40 immortalized human RCTEC/RCTE cells were characterized as being of distal tubule cell line as detailed in Loghman-Adham et al., 2003. On the basis of recent short tandem repeat (STR) DNA sequencing (Performed by IDEXX BioResearch) of the earliest passages of the RCTEC/RCTE cell stocks it became evident the cells were of mixed lineage. Further analyses of PCR products using QIAxcel capillary electrophoresis demonstrated the presence of a canine product. The product was sequenced and established to be of canine origin. A set of canine specific STR markers were compared to the sample and showed that the sample had a genetic profile with 92% identity to the MDCK cell line.  Later cell stocks that were used in the present publication were STR profiled and showed drift to 100% MDCK lineage. Despite being of canine origin, rather than human as was previously thought, MDCK is similarly a distal tubule epithelial cell line. For this reason, previous interpretation and conclusions drawn using these cells here and in Caralt et al., 2015, Uzarski et al., 2015 remain sound, but for purposes of rigor, reproducibility and experimental validation by others we report on this misidentification. This information will also be reported for listing on the International Cell Line Authentication Committee (ICLAC) database (http://iclac.org).

Résumé

An erratum was issued for Epithelial Cell Repopulation and Preparation of Rodent Extracellular Matrix Scaffolds for Renal Tissue Development.

The human RCTEC/RCTE cell stocks used here and in Caralt et al., 2015, Uzarski et al., 2015 were originally provided by Dr. Loghman-Adham (then at St. Louis University) to co-author Dr. Wandinger-Ness (University of New Mexico) through an MTA in 2001. The SV40 immortalized human RCTEC/RCTE cells were characterized as being of distal tubule cell line as detailed in Loghman-Adham et al., 2003. On the basis of recent short tandem repeat (STR) DNA sequencing (Performed by IDEXX BioResearch) of the earliest passages of the RCTEC/RCTE cell stocks it became evident the cells were of mixed lineage. Further analyses of PCR products using QIAxcel capillary electrophoresis demonstrated the presence of a canine product. The product was sequenced and established to be of canine origin. A set of canine specific STR markers were compared to the sample and showed that the sample had a genetic profile with 92% identity to the MDCK cell line.  Later cell stocks that were used in the present publication were STR profiled and showed drift to 100% MDCK lineage. Despite being of canine origin, rather than human as was previously thought, MDCK is similarly a distal tubule epithelial cell line. For this reason, previous interpretation and conclusions drawn using these cells here and in Caralt et al., 2015, Uzarski et al., 2015 remain sound, but for purposes of rigor, reproducibility and experimental validation by others we report on this misidentification. This information will also be reported for listing on the International Cell Line Authentication Committee (ICLAC) database (http://iclac.org).

Protocole

Une erratum a été publiée pour Repeuplement de cellules épithéliales et préparation de matrices extracellulaires de rongeur comme supports pour le développement de tissu rénal.

Les stocks cellulaires humains RCTEC/RCTE utilisés ici et dans Caralt et al. 2015, Uzarski et al. 2015 ont été initialement fournis par le Dr Loghman-Adham (alors à l'Université de Saint-Louis) au coauteur le Dr Wandinger-Ness (Université du Nouveau-Mexique) dans le cadre d'un MTA en 2001. Les cellules RCTEC/RCTE humaines immortalisées par le SV40 ont été caractérisées comme appartenant à une lignée de cellules du tube distal, comme décrit en détail dans Loghman-Adham et al. 2003. Sur la base de séquençages récents d'ADN par répétitions courtes en tandem (STR) (réalisés par IDEXX BioResearch) des premiers passages des stocks cellulaires RCTEC/RCTE, il est apparu que les cellules étaient d'une lignée mixte. Des analyses supplémentaires des produits PCR par électrophorèse capillaire sur QIAxcel ont révélé la présence d'un produit canin. Ce produit a été séquencé et identifié comme étant d'origine canine. Un ensemble de marqueurs STR spécifiques du chien a été comparé à l'échantillon et a montré que ce dernier possédait un profil génétique présentant une identité de 92 % avec la lignée cellulaire MDCK.  Les stocks cellulaires ultérieurs utilisés dans la présente publication ont été caractérisés par profilage STR et ont montré une dérive vers une lignée MDCK à 100 %. Bien qu'elles soient d'origine canine, et non humaine comme on le pensait précédemment, les cellules MDCK constituent également une lignée épithéliale de tube distal. Pour cette raison, les interprétations et conclusions antérieures établies à l'aide de ces cellules ici et dans Caralt et al. 2015, Uzarski et al. 2015 restent valables, mais par souci de rigueur, de reproductibilité et de validation expérimentale par d'autres chercheurs, nous signalons cette mauvaise identification. Cette information sera également communiquée pour être inscrite dans la base de données du Comité international pour l'authentification des lignées cellulaires (International Cell Line Authentication Committee, ICLAC) (http://iclac.org).