Erratum: Rearing the Fruit Fly Drosophila melanogaster Under Axenic and Gnotobiotic Conditions

Résumé

An erratum was issued for: Rearing the Fruit Fly Drosophila melanogaster Under Axenic and Gnotobiotic Conditions. The Protocol was updated.

Résumé

An erratum was issued for: Rearing the Fruit Fly Drosophila melanogaster Under Axenic and Gnotobiotic Conditions. The Protocol was updated.

Step 6.1.4.2 of the Protocol was updated from:

If using the 4 species described here, normalize cells to equivalent colony forming unit (CFU)/ml densities (OD₆₀₀ to CFU conversion determined previously²⁰) using this equation:
E = ((O-B) x V x D)/C
where E = volume to resuspend pellet in (μl), O = OD₆₀₀ bacteria, B = OD₆₀₀ blank media, D = fold-dilution, V = µl bacterial culture prior to centrifugation, C = OD₆₀₀ of predetermined constant. See Supplemental Code File for examples of calculations using these equations. For spectrophotometers that automatically blank, use "O" in place of "O-B".
Note: The predetermined constants (units OD₆₀₀, normalized to 10⁷ CFU ml^-1, constants derived in²⁰) are as follows: A. tropicalis (0.052), A. pomorum (0.038), L. brevis (0.056), L. plantarum (0.077).

to:

If using the 4 species described here, normalize cells to equivalent colony forming unit (CFU)/ml densities (OD₆₀₀ to CFU conversion determined previously²⁰) using this equation:
E = ((O-B) x V x D)/C
where E = volume to resuspend pellet in (μl), O = OD₆₀₀ bacteria, B = OD₆₀₀ blank media, D = fold-dilution, V = µl bacterial culture prior to centrifugation, C = OD₆₀₀ of predetermined constant. See Supplemental Code File for examples of calculations using these equations. For spectrophotometers that automatically blank, use "O" in place of "O-B".
Note: The predetermined constants (units OD₆₀₀, normalized to 10⁷ CFU ml^-1, constants derived in²⁰) are as follows: A. tropicalis (0.053), A. pomorum (0.038), L. brevis (0.077), L. plantarum (0.056).

Protocole

An erratum was issued for: Rearing the Fruit Fly Drosophila melanogaster Under Axenic and Gnotobiotic Conditions. The Protocol was updated.

Step 6.1.4.2 of the Protocol was updated from:

If using the 4 species described here, normalize cells to equivalent colony forming unit (CFU)/ml densities (OD₆₀₀ to CFU conversion determined previously²⁰) using this equation:
E = ((O-B) x V x D)/C
where E = volume to resuspend pellet in (μl), O = OD₆₀₀ bacteria, B = OD₆₀₀ blank media, D = fold-dilution, V = µl bacterial culture prior to centrifugation, C = OD₆₀₀ of predetermined constant. See Supplemental Code File for examples of calculations using these equations. For spectrophotometers that automatically blank, use "O" in place of "O-B".
Note: The predetermined constants (units OD₆₀₀, normalized to 10⁷ CFU ml^-1, constants derived in²⁰) are as follows: A. tropicalis (0.052), A. pomorum (0.038), L. brevis (0.056), L. plantarum (0.077).

to:

If using the 4 species described here, normalize cells to equivalent colony forming unit (CFU)/ml densities (OD₆₀₀ to CFU conversion determined previously²⁰) using this equation:
E = ((O-B) x V x D)/C
where E = volume to resuspend pellet in (μl), O = OD₆₀₀ bacteria, B = OD₆₀₀ blank media, D = fold-dilution, V = µl bacterial culture prior to centrifugation, C = OD₆₀₀ of predetermined constant. See Supplemental Code File for examples of calculations using these equations. For spectrophotometers that automatically blank, use "O" in place of "O-B".
Note: The predetermined constants (units OD₆₀₀, normalized to 10⁷ CFU ml^-1, constants derived in²⁰) are as follows: A. tropicalis (0.053), A. pomorum (0.038), L. brevis (0.077), L. plantarum (0.056).