High Flow Rate Stress
High Flow Rate Stress - Related Videos
13:35
Mass Conservation and Flow Rate Measurements
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2023
Source: Ricardo Mejia-Alvarez and Hussam Hikmat Jabbar, Department of Mechanical Engineering, Michigan State University, East Lansing, MI The purpose of this experiment is to demonstrate the calibration of a flow passage as a flowmeter using a control volume (CV) formulation [1, 2]. The CV analysis focuses on the macroscopic effect of flow on engineering systems, rather than the detailed description that could be achieved with a detailed differential analysis. These two techniques should be...
12:43
Parallel-plate Flow Chamber and Continuous Flow Circuit to Evaluate Endothelial Progenitor Cells under Laminar Flow Shear Stress
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Cited by 51 •
2012
We are describing a method to subject adherent cells to laminar flow shear stress in a sterile continuous flow circuit. The cells' adhesion, morphology can be studied through the transparent chamber, samples obtained from the circuit for metabolite analysis and cells harvested after shear exposure for future experiments or culture.
02:48
Zebrafish Rheotaxis Assay: A Method for Measuring Orientation Behavior in Response to Different Water Flow Rates
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2023
This video describes the rheotaxis assay in zebrafish. The method involves the measurement of orientation behavior of zebrafish in response to different water flow rates under the influence of different magnetic fields.
11:38
A Flow Adhesion Assay to Study Leucocyte Recruitment to Human Hepatic Sinusoidal Endothelium Under Conditions of Shear Stress
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Cited by 31 •
2014
Leucocyte recruitment to the liver occurs within the specialized channels of the hepatic sinusoids which are lined by unique hepatic sinusoidal endothelial cells. Phase contrast microscopy of leucocyte recruitment across human hepatic sinusoidal endothelium under conditions of physiological shear stress can facilitate the elucidation of the molecular mechanisms which underlie this process.
01:22
Renal Drug Excretion: Effect of Urine pH, Flow Rate, and Drug pKa
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2025
The pH of urine, the drug's pKa, and the urine flow rate are vital parameters for drug reabsorption and excretion. Urinary pH varies between 4.6 and 8.0 and is influenced by diet, drug intake, and the patient's pathophysiology. It affects a drug's ionization state and reabsorption. For instance, carbohydrate-rich food produces alkaline urine promoting drug excretion, while proteins and certain medications like ascorbic acid lead to acidic urine enhancing reabsorption. The pKa of a drug,...
FAQs
System geometry determines how rapidly fluid forces develop and where pressure gradients become concentrated, while material properties influence how cells, tissues, or engineered biomaterials respond. Consequently, the same flow rate may produce different changes in cell shape, adhesion, transport, or viability in different devices. Accounting for both factors is essential when interpreting results or designing a biological flow system.
Increasing flow can raise both fluid shear and pressure gradients, creating distinct mechanical conditions within a system. These forces may alter cell shape and adhesion while also changing transport through the experimental environment. Considering both effects helps researchers avoid treating flow rate as an isolated variable and improves evaluation of how physical conditions influence cultured cells, tissues, or biomaterials.
Flow must be controlled within a range that supports nutrient delivery and waste removal without producing excessive mechanical stress. Higher rates may improve transport but can also threaten cell or tissue viability, depending on system geometry and material properties. Adjusting and documenting flow conditions therefore helps researchers maintain biological function while reducing damage and improving experimental reproducibility.
Evaluation should relate the imposed flow rate to the system’s geometry, material properties, and observed biological responses. Useful outcomes include changes in cell shape, adhesion, transport, and viability, together with the effects on nutrient delivery and waste removal. This combined assessment shows whether a flow condition benefits the engineered system or introduces damaging mechanical effects.
Researchers investigate these effects in microfluidic devices, perfusion systems, and bioreactors by controlling fluid flow and examining its consequences for cells, tissues, or engineered biomaterials. The analysis can focus on physical and biological outcomes such as altered shape, adhesion, transport, and viability. Comparing responses under controlled flow conditions supports more reproducible experiments and informed system design.
Flow studies reveal how physical forces influence engineered tissues and cell cultures, including whether transport conditions support or compromise viability. These findings can guide the design of microfluidic devices, perfusion systems, and bioreactors by identifying how flow interacts with geometry and materials. The resulting knowledge supports improved experimental reproducibility and more effective tissue-engineering outcomes.