B2 Ordering

B2 ordering is a long-range atomic arrangement in which two elements preferentially occupy separate, interpenetrating simple-cubic sublattices, producing the ordered CsCl-type crystal structure. It forms when chemical interactions favor unlike-atom neighbors and thermal conditions allow atoms to occupy energetically preferred lattice sites; increasing temperature can reduce this order and promote a disordered state. In engineering materials, B2 ordering is important in intermetallic compounds and alloys such as NiAl and FeAl, where it influences phase stability, strength, ductility, diffusion, and oxidation behavior. Characterizing B2 order helps researchers design alloys with controlled structural and high-temperature performance.

B2 Ordering - Related Videos

Research

JoVE Journal - Engineering
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Sputter Growth and Characterization of Metamagnetic B2-ordered FeRh Epilayers

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Cited by 23 •

2013

A method to prepare epitaxial layers of ordered alloys by sputtering is described. The B2-ordered FeRh compound is used as an example, as it displays a metamagnetic transition that depends sensitively on the degree of chemical order and the exact composition of the alloy.

Research

JoVE Journal - Developmental Biology

Dissection and Culture of Mouse Embryonic Kidney

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Cited by 4 •

2017

This protocol describes a method for isolating and culturing metanephric rudiments from mouse embryos.

An Assay to Evaluate Drug Efficacy against a Mycobacterium tuberculosis Infection Model

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2025

This video demonstrates an assay to evaluate the efficacy of drugs against Mycobacterium tuberculosis (Mtb) infection. An in vitro infection model containing Mtb/macrophage aggregates is generated that mimics in vivo infection. Upon introducing suitable antibiotics, their efficacy is tested via resuzarin assay to monitor the survival of bacteria with increasing drug concentrations.

Isolation of Proteins from Viral DNA-protein Complexes

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2026

Source: Dembowski, J. A., et al. Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins. J. Vis. Exp. (2017).This video demonstrates the isolation of protein–viral DNA complexes from cell nuclei using click chemistry and magnetic bead purification. The protocol involves labeling viral genomes with alkyne-modified nucleotides, biotinylation via a click reaction, and binding to streptavidin-coated magnetic beads. Following washing and heat elution, the purified...

In Vitro Evaluation of Bacterial Killing by Neonatal Mouse Splenic Phagocytes

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2026

Source: Seman, B. G., et.al. Neonatal Imaging Model of Gram-Negative Bacterial Sepsis. J. Vis. Exp. (2020)This video demonstrates the isolation of neutrophils and inflammatory monocytes from neonatal mouse spleens and their application in a luminescent bacterial killing assay. It covers mechanical dissociation, red blood cell lysis, magnetic bead–based cell enrichment, bacterial incubation, and antibiotic protection. Timed luminescence readings indicate initial bacterial internalization...

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