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Isolation and Enrichment of Rat Mesenchymal Stem Cells (MSCs) and Separation of Single-colony Derived MSCs

Article DOI: 10.3791/1852-v • 08:19 min • March 22nd, 2010
March 22nd, 2010 •
1, 1
1Department of Chemical Engineering and Materials Science, City of Hope Cancer Center

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Rat MSCs were isolated from femurs and tibias and then enriched by magnetic cell sorting. Sorted cells were confirmed for the expression of surface markers by flow cytometry. These cells were also cultured at clonal density to form single colonies and then these colonies were separated by cloning cylinders.

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Isolation Enrichment Rat Mesenchymal Stem Cells MSCs Single-colony Derived MSCs Bone Marrow Hematopoietic Stem Cells Plastic Adherence Protocol Rat Femurs Tibias Magnetic Cell Sorting Surface Markers CD54 CD90 Flow Cytometry Analysis HSC Marker CD45 Heterogeneity Subpopulations Cloning Cylinders Culture Evaluation
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