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Journal / Genetics / A Guide to Precise Gene Disruption in Primary… / 用于小鼠骨髓来源巨噬细胞基因组编辑的电穿孔和核糖核蛋白递送的制备…
JoVE Journal
Genetics

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JoVE Journal Genetics
用于小鼠骨髓来源巨噬细胞基因组编辑的电穿孔和核糖核蛋白递送的制备
Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells
Journal (Genetics)
Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells
Systemic Delivery of MicroRNA Using Recombinant Adeno-associated Virus Serotype 9 to Treat Neuromuscular Diseases in Rodents
Journal (Genetics)
Systemic Delivery of MicroRNA Using Recombinant Adeno-associated Virus Serotype 9 to Treat Neuromuscular Diseases in Rodents
 
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用于小鼠骨髓来源巨噬细胞基因组编辑的电穿孔和核糖核蛋白递送的制备

A Guide to Precise Gene Disruption in Primary Bone Marrow-Derived Macrophages

Article DOI: 10.3791/200896-v • 04:03 min • August 4th, 2023
August 4th, 2023 •
1, 1, 1,2
1Department of Internal Medicine, University of California, Davis, 2Department of Medical Microbiology and Immunology, University of California, Davis

Tags

本月在JoVE，第198期，电穿孔，Cas9-sgRNA复合物，CRISPR/Cas9基因组编辑，移码突变，小插入和缺失（插入缺失），基因功能，单向导RNA（sgRNA），核糖核蛋白复合物（RNP），编辑效率，Sanger测序，分析程序
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