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In vivo Quantification of G Protein Coupled Receptor Interactions using Spectrally Resolved Two-photon Microscopy

Article DOI: 10.3791/2247-v • 14:26 min • January 19th, 2011
January 19th, 2011 •
1, 1, 1,2
1Department of Physics, University of Wisconsin - Milwaukee, 2Department of Biological Sciences, University of Wisconsin - Milwaukee

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By employing a spectrally resolved two-photon microscopy imaging system, pixel-level maps of Förster Resonance Energy Transfer (FRET) efficiencies are obtained for cells expressing membrane receptors hypothesized to form homo-oligomeric complexes. From the FRET efficiency maps, we are able to estimate stoichiometric information about the oligomer complex under study.

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In Vivo Quantification G Protein Coupled Receptor Interactions Spectrally Resolved Two-photon Microscopy Protein Interactions FRET Fluorescent Probes Living Cells Industrial Applications Basic Biological Knowledge Donor Molecule Acceptor Molecule Laser Light Fluorescence Emission Spectral Properties Protein Interactions Degree Cell Scanning Molecular Composition Acquisition Process Spatial Determination Quantitative Values Spectrally Resolved Two-photon Microscope
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