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Patch Clamp and Perfusion Techniques for Studying Ion Channels Expressed in Xenopus oocytes

Article DOI: 10.3791/2269-v • 10:19 min • January 10th, 2011
January 10th, 2011 •
1, 2, 2, 3
1Department of Energy, Environmental & Chemical Engineering, Washington University in St. Louis, 2Department of Biomedical Engineering, Washington University in St. Louis, 3Department of Biomedical Engineering and Cardiac Bioelectricity and Arrhythmia Center, Washington University in St. Louis

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Ionic current of BK channels is recorded using patch clamp techniques. BK channels are expressed in Xenopus oocytes by injecting messenger RNA. The intracellular solution during patch clamp recordings is controlled by a perfusion system.

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Patch Clamp Perfusion Techniques Ion Channels Xenopus Oocytes BK Channels Activation Structure-function Relationship Neurotransmitter Receptors Smooth Muscle Contraction Hair Cell Frequency Tuning Neurotransmitter Release Membrane Depolarization Intracellular Ca2+ And Mg2+ Messenger RNA Injection Incubation Inside-out Configuration Single Or Multiple Channels Recording Currents Controlled Voltage
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