This content is Free Access.
Chapitres
- 00:05Titre
- 01:30Formaldehyde Fixation of Long-Range Chromatin Interactions and Nuclei Isolation
- 03:36Digestion, Ligation and Purification of Interaction DNA Segments
- 06:10Digestion with Msp I and Ligation with Oligonucleotide Linkers
- 07:50PCR Amplification and Sequence Analysis
- 11:06Act Assay Representative Results
- 14:23Conclusion
The associated chromosome trap (ACT) assay is a novel unbiased method for identifying long-range DNA interactions. The characterization of long range DNA interactions will allow us to determine the relationship of nuclear architecture to gene expression in both normal physiology and in diseased states.
