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Neural-Colony Forming Cell Assay: An Assay To Discriminate Bona Fide Neural Stem Cells from Neural Progenitor Cells

Article DOI: 10.3791/2639-v • 06:31 min • March 6th, 2011
March 6th, 2011 •
1,2, 3, 1, 1, 1
1Department of Neurosurgery, University of Florida, 2Department of Anatomical Sciences, Shiraz University of Medical Sciences, 3STEMCELL Technologies, Inc.

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This video protocol demonstrates how to discriminate and enumerate bona fide neural stem cells in a mixed population of neural precursor cells using the neural colony-forming cell assay.

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Neural-Colony Forming Cell Assay Assay Discriminate Neural Stem Cells Neural Progenitor Cells Neurosphere Assay Isolate Expand Frequency Serum-free Culture System Tumors Normal Tissues, Neurosphere Formation Overestimates Mixed Population Embryonic Brain Adult Mammalian Brain Collagen-based Semi-solid Assay N-CFCA Proliferative Potential Enumerate NSC Frequency Colonies Functional Criteria Primary Cells Cultured Cells
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