Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy

The reverse genetics system for the Rift Valley fever virus MP-12 vaccine strain is a useful tool for creating additional MP-12 mutants with increased attenuation and immunogenicity. We describe the protocol to generate and characterize NSs mutant strains.

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Reverse Genetics NSs Gene Rift Valley Fever Virus MP-12 Strain Vaccine Safety Vaccine Efficacy Hemorrhagic Fever Neurological Disorders Blindness Abortion Fetal Malformation HHS/USDA Overlap Select Agent Risk Group 3 Pathogen Phlebovirus Bunyaviridae Family Virulent RVFV MP-12 Vaccine Strain Wild-type RVFV Strains ZH548 ZH501 M-segment L-segment S-segment RNA3 Functional Virulence Factor NSs RMP12-C13type In-frame Deletion Of NSs ORF Host Transcription Shut-off Interferon (IFN)-beta MRNA7,8 Double-stranded RNA-dependent Protein Kinase (PKR) Post-translational Level Degradation Of PKR

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Journal (Immunology and Infection)

Journal (Immunology and Infection)

Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy

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