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Immunology and Infection

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JoVE Journal Immunology and Infection
Locked Nucleic Acid Flow Cytometry-fluorescence in situ Hybridization (LNA flow-FISH)
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Locked Nucleic Acid Flow Cytometry-fluorescence in situ Hybridization (LNA flow-FISH): a Method for Bacterial Small RNA Detection

Article DOI: 10.3791/3655-v • 09:46 min • January 10th, 2012
January 10th, 2012 •
1, 1
1Center for Bio/Molecular Science and Engineering, Naval Research Laboratory

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résumé
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A novel high-throughput method is described that enables the detection and relative quantitation of small RNA and mRNA expression from single bacterial cells using locked nucleic acid probes and flow cytometry-fluorescence in situ hybridization.

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Locked Nucleic Acid Flow Cytometry Fluorescence In Situ Hybridization FISH Nucleic Acid Sequences Flow-FISH Lysate-based Nucleic Acid Detection Methods Statistical Analysis Variance Analysis Telomere Length Determination Cellular Identification Gene Expression Viral Multiplication Bacterial Community Analysis DNA Oligonucleotide Probes Nucleic Acid Analogs Sensitivity Specificity Melting Temperatures (Tm) Locked Nucleic Acid (LNA) Probes
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