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JoVE Journal Neuroscience
Recording Electrical Activity from Identified Neurons in the Intact Brain of Transgenic Fish
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Recording Electrical Activity from Identified Neurons in the Intact Brain of Transgenic Fish

Article DOI: 10.3791/50312-v • 07:45 min • April 30th, 2013
April 30th, 2013 •
1, 1
1Department of Physiology, University of California, Los Angeles

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In this video, we will demonstrate how to record electrical activity from identified single neurons in a whole brain preparation, which preserves complex neural circuits. We use transgenic fish in which gonadotropin-releasing hormone (GnRH) neurons are genetically tagged with a fluorescent protein for identification in the intact brain preparation.

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Electrical Activity Identified Neurons Intact Brain Transgenic Fish Cell Physiology Neural Circuits Model Systems Intact Brain Preparation CNS Transgenic Fish Gonadotropin-releasing Hormone (GnRH) Neurons Green Fluorescent Protein Identification Multiple Populations Location In The Brain GnRH Gene Terminal Nerves (TN) Olfactory Bulbs Medaka Fish Neuromodulatory Neurons Transmitter Of Information Central Nervous System Pituitary-gonadal Functions Hypothalamic GnRH1 Neurons Spontaneous Action Potential Firing Tonic Pattern Visual Cues Conspecifics, Neuropeptide Kisspeptin
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