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Prostaglandin Extraction and Analysis in Caenorhabditis elegans
Journal JoVE
Biologie
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Journal JoVE Biologie
Prostaglandin Extraction and Analysis in Caenorhabditis elegans

Prostaglandin Extraction and Analysis in Caenorhabditis elegans

DOI:
10.3791/50447-v

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08:50 min

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June 25, 2013

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, , ,
1Department of Pharmacology and Toxicology,University of Alabama at Birmingham, 2Department of Cell, Developmental, and Integrative Biology,University of Alabama at Birmingham

Chapitres

  • 00:05Titre
  • 01:05Worm Culture
  • 03:28Prostaglandin Extraction
  • 06:14Prostaglandin Analysis
  • 07:15Results: Prostaglandin Extraction and Analysis in C. elegans
  • 08:22Conclusion

Summary

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In this paper, we describe an optimized procedure for extracting and analyzing prostaglandins and other eicosanoids from C. elegans using LC-MS/MS.

Tags

ProstaglandinCaenorhabditis ElegansEicosanoidLipid ExtractionLiquid Chromatography-mass SpectrometryTandem Mass SpectrometryMultiple Reaction MonitoringCollision-induced Decomposition

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