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Neuroscience

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JoVE Journal Neuroscience
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Visualizing the Effects of a Positive Early Experience, Tactile Stimulation, on Dendritic Morphology and Synaptic Connectivity with Golgi-Cox Staining

Article DOI: 10.3791/50694-v • 08:43 min • September 25th, 2013
September 25th, 2013 •
1, 1, 1
1Canadian Centre for Behavioural Neuroscience, University of Lethbridge

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This paper describes the procedures for tactile stimulation of rat pups and subsequent Golgi-Cox staining of neuronal morphology. Tactile stimulation is a positive experience that is administered in the perinatal period by stroking pups with a household duster. Golgi-cox staining is a reliable procedure permitting the visualization of entire neurons.

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Visualizing Effects Positive Early Experience Tactile Stimulation Dendritic Morphology Synaptic Connectivity Golgi-Cox Staining Neuronal Changes Rat Pups Cross-litter Design Neuroplastic Changes Brain Tissue Visualization Golgi-Cox Solution Staining Procedure Entire Neuronal Elements Dendrites Axons Dendritic Spines Ongoing Analysis
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