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Cell-based Assay Protocol for the Prognostic Prediction of Idiopathic Scoliosis Using Cellular Dielectric Spectroscopy

Article DOI: 10.3791/50768-v • 08:08 min • October 16th, 2013
October 16th, 2013 •
1, 1, 1,2
1Viscogliosi Laboratory in Molecular Genetics of Musculoskeletal Diseases, Sainte-Justine University Hospital Research Center, 2Department of Stomatology, Faculty of Dentistry and Department of Biochemistry, Faculty of Medicine, Université de Montréal

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A structured protocol is presented for a cell-based assay as a functional test to predict the prognosis of idiopathic scoliosis using cellular dielectric spectroscopy (CDS). The assay can be performed with fresh or frozen peripheral blood mononuclear cells (PBMCs) and the procedure is completed within 4 days.

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Cell-based Assay Prognostic Prediction Idiopathic Scoliosis Cellular Dielectric Spectroscopy Functional Test Peripheral Blood Mononuclear Cells Gi And Gs Proteins Automated CDS-based Instrument Functional Groups Osteopontin Disease Progression Ficoll-gradient Cell Culture Phytohemmaglutinin (PHA) Somatostatin Isoproterenol
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