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Videomorphometric Analysis of Hypoxic Pulmonary Vasoconstriction of Intra-pulmonary Arteries Using Murine Precision Cut Lung Slices
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Videomorphometric Analysis of Hypoxic Pulmonary Vasoconstriction of Intra-pulmonary Arteries Using Murine Precision Cut Lung Slices

Article DOI: 10.3791/50970-v • 13:32 min • January 14th, 2014
January 14th, 2014 •
1, 1, 1, 1
1Institute of Anatomy and Cell Biology, Justus-Liebig-University

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Hypoxic pulmonary vasoconstriction (HPV) is an important physiological phenomenon by which at alveolar hypoxia lung perfusion is matched to ventilation. The major vascular segment contributing to HPV is the intra-acinar artery. Here, we describe our protocol for the analysis of HPV of murine pulmonary vessels with diameters of 20-100 μm.

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Videomorphometric Analysis Hypoxic Pulmonary Vasoconstriction Intra-pulmonary Arteries Murine Precision Cut Lung Slices Acute Alveolar Hypoxia Von Euler-Liljestrand Mechanism Lung Perfusion Ventilation Underlying Mechanisms Major Vascular Segment Intra-acinar Artery Blood Supply Acinus Terminal Bronchiole Measurement Techniques Pulmonary Artery Pressure Force Recordings Subpleural Vessels Confocal Laser Scanning Luminescence Microscopy Technique Murine Intra-pulmonary Arteries Inner Diameter Vasoreactivity
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