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Ablation of a Single Cell From Eight-cell Embryos of the Amphipod Crustacean Parhyale hawaiensis

Article DOI: 10.3791/51073-v • 10:55 min • March 16th, 2014
March 16th, 2014 •
1, 1
1Department of Organismic and Evolutionary Biology, Harvard University

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The amphipod Parhyale hawaiensis is a promising model organism for studies of crustacean embryology and comparative arthropod development and evolution. This protocol describes a method for manual removal of single blastomeres from early cleavage stage embryos of Parhyale.

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Ablation Single Cell Eight-cell Embryos Amphipod Crustacean Parhyale Hawaiensis Model Organism Development Outgroup Comparison Arthropod Drosophila Melanogaster Syncytial Cleavages Holoblastic Cleavages Fate Mapping Germ Layers Germ Line Ectoderm Mesoderm Endoderm Blastomere Ablation Regulatory Capabilities Phototoxic Dyes Manual Ablation Physical Removal
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