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Dissection of the Transversus Abdominis Muscle for Whole-mount Neuromuscular Junction Analysis
Journal JoVE
Neurosciences
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Journal JoVE Neurosciences
Dissection of the Transversus Abdominis Muscle for Whole-mount Neuromuscular Junction Analysis

Dissection of the Transversus Abdominis Muscle for Whole-mount Neuromuscular Junction Analysis

DOI:
10.3791/51162-v

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06:12 min

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January 11, 2014

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, ,
1Regenerative Medicine Program,Ottawa Hospital Research Institute, 2Centre for Integrative Physiology,University of Edinburgh

Chapitres

  • 00:00Titre
  • 00:34Dissection of Abdominal Musculature from a Recently Euthanized Mouse
  • 02:15Isolation of the TVA Muscle
  • 04:17Immunofluorescent Labeling and Microscopy of the TVA Muscle
  • 05:04Conclusions and Results

Summary

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In this video we demonstrate a protocol for dissection of the transversus abdominis muscle of the mouse and use immunofluorescence and microscopy to visualize neuromuscular junctions.

Tags

Transversus AbdominisNeuromuscular JunctionMotor NeuronDissectionImmunofluorescenceSpinal Muscular AtrophyMotor UnitAxonal BranchingTerminal SproutingInnervation Pattern

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