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Chapitres
- 00:05Titre
- 01:13Generation of Recombinant Baculovirus
- 04:57Amplification of Recombinant Baculovirus
- 06:23Expression of Kindlin-3 in Baculovirus-infected Sf9
- 07:39Purification of Recombinant Kindlin-3
- 09:33Results: Milligram Quantitites of Recombinant Murine Kindlin-3 Produced from Insect Cells
- 13:21Conclusion
Kindlins are fundamental to cell adhesion through integrins but studies of them have been hampered by the difficulty encountered in expressing them recombinantly in bacterial hosts. We describe here methods for their efficient production in baculovirus-infected insect cells.
