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Isolation and Culture of Dental Epithelial Stem Cells from the Adult Mouse Incisor

Article DOI: 10.3791/51266-v • 08:14 min • May 1st, 2014
May 1st, 2014 •
1,2, 1, 1, 1,3, 1, 4,5,6, 1,7, 1,8
1Department of Orofacial Sciences and Program in Craniofacial and Mesenchymal Biology, University of California, San Francisco, 2Department of Bioengineering and Therapeutic Sciences, University of California, San Francisco, 3Department of Pathology and Research Center, Zhongshan Hospital of Dalian University, 4Université Paris Descartes, Sorbonne Paris Cite, UMR S872, 5Centre de Recherche des Cordeliers, Université Pierre et Marie Curie, UMR S872, 6INSERM U872, 7Division of Endodontics, Department of Preventive and Restorative Dental Sciences, University of California, San Francisco, 8Department of Pediatrics and Institute for Human Genetics, University of California, San Francisco

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résumé
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The continuously growing mouse incisor provides a model for studying renewal of dental tissues from dental epithelial stem cells (DESCs). A robust system for consistently and reliably obtaining these cells from the incisor and expanding them in vitro is reported here.

Tags

Dental Epithelial Stem Cells Adult Mouse Incisor Tooth Regeneration Cellular Mechanisms Molecular Mechanisms Dental Stem Cells Labial Cervical Loop Lingual Cervical Loop Ameloblasts Enamel Formation Dental Tissues Progenitor Cells Stem Cell Culture
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