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JoVE Journal Bioengineering
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Expression, Isolation, and Purification of Soluble and Insoluble Biotinylated Proteins for Nerve Tissue Regeneration

Article DOI: 10.3791/51295-v • 12:03 min • January 22nd, 2014
January 22nd, 2014 •
1, 1, 1, 1
1Department of Chemical and Biomolecular Engineering, University of Akron

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Developing biotinylatable fusion proteins has many potential applications in various fields of research. Recombinant protein engineering is a straight forward procedure that is cost-effective, providing high yields of custom-designed proteins.

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Expression Isolation Purification Soluble Biotinylated Proteins Insoluble Biotinylated Proteins Nerve Tissue Regeneration Recombinant Protein Engineering E. Coli Expression Systems Biotin Tag Green Fluorescent Protein Cherry Red Protein Unnatural Amino Acids Metal Ion Chelators Functional Groups Spectroscopic Probes Post-translational Modifications Fusion Proteins Target Proteins Avidin-streptavidin Interaction Detection And Purification Of Tagged Proteins Cell Sorting Biotin-labeled Molecules Bioindustrial Applications Biomedical Applications Nerve Growth Factor (NGF) Semaphorin3A (Sem)
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