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RNAscope for In situ Detection of Transcriptionally Active Human Papillomavirus in Head and Neck Squamous Cell Carcinoma
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RNAscope for In situ Detection of Transcriptionally Active Human Papillomavirus in Head and Neck Squamous Cell Carcinoma

Article DOI: 10.3791/51426-v • 10:26 min • March 11th, 2014
March 11th, 2014 •
1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1
1Advanced Cell Diagnostics, Inc.

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The presence of high-risk HPV in head and neck tumor tissue is associated with favorable outcomes. The recently developed RNA in situ hybridization technique called RNAscope allows direct visualization of HPV E6/E7 mRNA in FFPE tissue sections.

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RNAscope In Situ Detection Transcriptionally Active Human Papillomavirus Head And Neck Squamous Cell Carcinoma Oncogenic HPV E6/E7 MRNA Quantitative Reverse Transcription Polymerase Chain Reaction (qRT-PCR) RNA Extraction Tumor Tissue Context Morphological Correlation Routine Clinical Practice RNA In Situ Hybridization Technology Formalin-fixed Paraffin-embedded (FFPE) Tissue Single Molecule Sensitivity Single Cell Resolution RNA Biomarker HPV Assay High-risk HPV Genotypes Sensitivity And Specificity Gold Standard Method Prognostic Clinical Outcome Oropharyngeal Cancer Patients
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