Using the Overlay Assay to Qualitatively Measure Bacterial Production of and Sensitivity to Pneumococcal Bacteriocins

Natalie Maricic; Suzanne Dawid

doi:10.3791/51876

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Utilisation de l'Essai de superposition pour mesurer qualitativement la production bactérienne et la sensibilité à pneumocoque bactériocines

Using the Overlay Assay to Qualitatively Measure Bacterial Production of and Sensitivity to Pneumococcal Bacteriocins

Journal JoVE
Immunologie et infection

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Journal JoVE Immunologie et infection

Using the Overlay Assay to Qualitatively Measure Bacterial Production of and Sensitivity to Pneumococcal Bacteriocins

Utilisation de l'Essai de superposition pour mesurer qualitativement la production bactérienne et la sensibilité à pneumocoque bactériocines

Please note that all translations are automatically generated. Click here for the English version.

13,547 Views

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06:05 min

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September 30, 2014

DOI:

10.3791/51876-v

DOI:

10.3791/51876-v

•

06:05 min

September 30, 2014

•

13525 Views

Natalie Maricic¹, Suzanne Dawid^1,2

¹Microbiology and Immunology,University of Michigan ²Pediatrics and Communicable Diseases,University of Michigan

Summary

Competition in Streptococcus pneumoniae is mediated by bacteriocins, small antimicrobial peptides with inhibitory activity towards pneumococcus and other related species. Here we describe an optimized bacterial overlay assay that allows for the characterization of bacteriocin activity and inhibitory spectrum, bacteriocin-specific immunity, and detection of secreted quorum sensing peptides.