Construction of an Affordable and Easy-to-Build Zebrafish Facility

Candler Paige; Bailey Hill; Joseph Canterbury; Sarah Sweitzer; E. Alfonso Romero-Sandoval

doi:10.3791/51989

Construction d'un Construire facile à l'installation de poisson zèbre et abordable

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Biology

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JoVE Journal Biology

Construction of an Affordable and Easy-to-Build Zebrafish Facility

Construction d'un Construire facile à l'installation de poisson zèbre et abordable

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12:19 min

November 22, 2014

DOI: 10.3791/51989-v

Candler Paige¹, Bailey Hill¹, Joseph Canterbury¹, Sarah Sweitzer¹, E. Alfonso Romero-Sandoval¹

¹Department of Administration and Pharmaceutical Sciences,Presbyterian College School of Pharmacy

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Overview

This article describes the construction of an affordable zebrafish facility designed for breeding and in vivo research. The facility requires minimal maintenance and is suitable for student involvement and independent research.

Key Study Components

Area of Science

Neuroscience
Biology
Animal Research

Background

Zebrafish are widely used in biological research due to their transparent embryos and rapid development.
Building a dedicated facility can enhance research capabilities at small institutions.
Cost-effective solutions are essential for educational settings.
Minimal maintenance is crucial for sustainability in research environments.

Purpose of Study

To provide a step-by-step guide for constructing a zebrafish facility.
To optimize the facility for various zebrafish research techniques.
To encourage student participation in hands-on research.

Methods Used

Layout and assembly of PVC components for the facility.
Installation of water supply lines and exhaust systems.
Conducting a 72-hour test run to ensure system functionality.
Optimization of water quality for zebrafish health.

Main Results

The facility was successfully constructed using affordable materials.
Minimal maintenance requirements were achieved.
The system was tested and optimized for fish welfare.
Student involvement was facilitated through hands-on construction.

Conclusions

The zebrafish facility serves as a model for small institutions.
Cost-effective research setups can enhance educational opportunities.
Proper planning and assembly lead to successful research environments.

Frequently Asked Questions

What materials are needed to build the zebrafish facility?

PVC pipes, a reservoir, a pump, and filters are essential for construction.

How long does it take to set up the facility?

The setup can be completed in a few hours, followed by a 72-hour test run.

Is the facility suitable for educational purposes?

Yes, it is designed to involve students in practical research activities.

What is the importance of water quality in the facility?

Maintaining optimal water quality is crucial for the health and welfare of the zebrafish.

Can this facility be adapted for other types of fish?

While designed for zebrafish, the principles can be adapted for other species with similar needs.

What are the benefits of using zebrafish in research?

Zebrafish are transparent during early development, allowing for easy observation of biological processes.

Une installation de poisson zèbre adaptée à la reproduction et à la recherche in vivo est construite de manière simple et abordable. L’entretien est minimal. Cette installation est idéale pour l’implication des étudiants et la recherche indépendante.

L’objectif global de cette procédure est de construire une installation abordable pour les poissons-zèbres qui nécessite un entretien minimal. Pour ce faire, il suffit d’abord de disposer les pièces en PVC, le réservoir, la pompe et les filtres et de les assembler à l’aide d’un apprêt et d’une colle pour PVC avant de les fixer à l’étagère. La deuxième étape consiste à assembler les conduites d’alimentation de chacune des trois étagères, en veillant à inclure une conduite de décompression.

Ensuite, les conduites d’échappement de l’aquarium sont construites et connectées à une conduite d’échappement principale. La dernière étape consiste à préparer le système pour les poissons en effectuant un essai de 72 heures et en optimisant la qualité de l’eau. En fin de compte, cette installation est optimisée pour une variété de techniques de recherche sur le poisson zèbre dans une petite institution.

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