Transposon Mediated intégration du plasmide ADN dans le zone sous-ventriculaire de souris néonatale pour générer des modèles nouveaux de glioblastome
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February 22, 2015
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Here we describe an efficient and versatile protocol to induce, monitor and analyze novel glioblastomas (GBM) using transposon DNA injected into the ventricles of neonatal mice. Cells of the subventricular zone, which take up the plasmid, transform, proliferate and generate tumors with histo-pathological characteristics of human GBM.
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Calinescu, A., Núñez, F. J., Koschmann, C., Kolb, B. L., Lowenstein, P. R., Castro, M. G. Transposon Mediated Integration of Plasmid DNA into the Subventricular Zone of Neonatal Mice to Generate Novel Models of Glioblastoma. J. Vis. Exp. (96), e52443, doi:10.3791/52443 (2015).
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