Comprehensive Assessment of Germline Chemical Toxicity Using the Nematode <em>Caenorhabditis elegans</em>

Daniela A. Parodi; Robert Damoiseaux; Patrick Allard

doi:10.3791/52445

Journal

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Biologie du développement

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Évaluation complète de la lignée germinale toxicité chimique Utilisation du nématode<em> Caenorhabditis elegans</em

Comprehensive Assessment of Germline Chemical Toxicity Using the Nematode <em>Caenorhabditis elegans</em>

Journal JoVE
Biologie du développement

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Journal JoVE Biologie du développement

Comprehensive Assessment of Germline Chemical Toxicity Using the Nematode Caenorhabditis elegans

Évaluation complète de la lignée germinale toxicité chimique Utilisation du nématode<em> Caenorhabditis elegans</em

Please note that all translations are automatically generated. Click here for the English version.

7,979 Views

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10:55 min

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February 22, 2015

DOI:

10.3791/52445-v

DOI:

10.3791/52445-v

•

10:55 min

February 22, 2015

•

2 Views

Daniela A. Parodi¹, Robert Damoiseaux³, Patrick Allard^1,2

¹Institute for Society and Genetics,University of California, Los Angeles, ²Environmental Health Sciences,University of California, Los Angeles, ³California Nanosystems Institute, Department of Molecular and Medical Pharmacology,University of California, Los Angeles

Summary

We describe the detailed steps of a high-throughput chemical assay in the nematode Caenorhabditis elegans used to assess germline toxicity. In this assay, disruption of germline function following chemical exposure is monitored using a fluorescent reporter specific to aneuploid embryos.