Th17 Inflammation Model of Oropharyngeal Candidiasis in Immunodeficient Mice

Natarajan Bhaskaran; Aaron Weinberg; Pushpa Pandiyan

doi:10.3791/52538

Th17 Inflammation modèle de la candidose oropharyngée chez des souris immunodéficientes

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Th17 Inflammation Model of Oropharyngeal Candidiasis in Immunodeficient Mice

Th17 Inflammation modèle de la candidose oropharyngée chez des souris immunodéficientes

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08:02 min

February 18, 2015

DOI: 10.3791/52538-v

Natarajan Bhaskaran¹, Aaron Weinberg¹, Pushpa Pandiyan¹

¹Department of Biological Sciences, School of Dental Medicine,Case Western Reserve University

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Overview

This study presents a method to establish Th17 immunopathology associated with oral Candida infection in immunodeficient mice. The model aims to investigate T cell mediated immunopathology in the context of Candida infection.

Key Study Components

Area of Science

Immunology
Microbiology
Pathology

Background

Candida infections pose significant health challenges.
Existing models do not adequately study T cell responses.
Th17 cells are implicated in various immunopathologies.
Understanding these mechanisms can inform therapeutic strategies.

Purpose of Study

To develop a model of oral inflammation mediated by Th17 cells.
To assess the role of Th17 cells in Candida infection.
To explore implications for oral immunotherapy.

Methods Used

Injection of Th17 cells into immunodeficient mice.
Culture and preparation of Candida albicans blasto spores.
Oral injection of blasto spores to induce inflammation.
Analysis of weight loss and tongue inflammation via flow cytometry and immunohistochemistry.

Main Results

Mice injected with Th17 cells exhibited significant weight loss.
Inflammation of the tongue was observed in these mice.
Flow cytometry and immunohistochemistry confirmed the findings.
The model demonstrates the pathogenic role of Th17 cells in oral Candida infection.

Conclusions

This model provides insights into T cell mediated immunopathology.
It has potential applications in developing oral immunotherapies.
Further research can expand understanding of Candida infections.

Frequently Asked Questions

What is the significance of Th17 cells in this study?

Th17 cells are central to the immunopathology observed in Candida infections, making them a key focus for understanding disease mechanisms.

How does this model contribute to immunotherapy?

By elucidating the role of Th17 cells, this model may inform strategies for oral immunotherapy against Candida infections.

What methods were used to analyze inflammation?

Flow cytometry and immunohistochemistry were employed to assess inflammation and immune responses in the tongue of the mice.

What are the implications of weight loss in the mice?

Weight loss indicates a systemic response to infection and inflammation, highlighting the severity of the immunopathology induced by Th17 cells.

Can this model be used for other types of infections?

While this model is specific to Candida, the approach may be adapted to study other infections involving T cell mediated responses.

Bien qu’il existe des modèles d’infection à Candida pour étudier la résistance immunitaire de l’hôte, il n’existe aucun modèle pour étudier l’immunopathologie induite par les lymphocytes T dans le contexte de l’infection à Candida. Nous décrivons ici une méthode pour établir l’immunopathologie Th17 associée à l’infection orale à Candida chez les souris immunodéficientes.

L’objectif global de cette procédure est de développer un modèle d’inflammation buccale dépendante de l’infection qui est médiée par les cellules pathologiques TH 17. Ceci est accompli en injectant d’abord ces 17 cellules dans des souris immunodéficientes. L’étape suivante consiste à cultiver le champignon candida albicans, à collecter les spores de blasto et à les préparer dans PBS.

La dernière étape consiste à injecter oralement les souris avec les spores de blasto pour stimuler la prolifération des cellules 17 injectées et induire une inflammation buccale. Les résultats montrent que les souris injectées de TH 17 succombent à la perte de poids et à l’inflammation de la langue sur la base de la cytométrie en flux et de l’analyse immunohistochimique de la langue. Ainsi, les implications de cette technique s’étendent à l’immunothérapie orale en tant que modèle murin.

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