Synchronization of bacterial cells is essential for studies of the bacterial cell cycle and development. Caulobacter crescentus is synchronizable through density centrifugation allowing a rapid and powerful tool for studies of the bacterial cell cycle. Here we provide a detailed protocol for the synchronization of Caulobacter cells.
Schrader, J. M., Shapiro, L. Synchronization of Caulobacter Crescentus for Investigation of the Bacterial Cell Cycle. J. Vis. Exp. (98), e52633, doi:10.3791/52633 (2015).