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Bioengineering

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Procedure for Decellularization of Rat Livers in an Oscillating-pressure Perfusion Device

Article DOI: 10.3791/53029-v • 09:38 min • August 10th, 2015
August 10th, 2015 •
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1General, Visceral, and Transplantation Surgery, Charité – Universitätsmedizin Berlin

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The presented techniques for liver harvesting, cannulation and perfusion using our proprietary device enable sophisticated perfusion set-ups to improve decellularization and recellularization experiments in rat livers.

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Decellularization Rat Livers Oscillating-pressure Perfusion Device Perfusion Portal Vein Hepatic Artery Homogeneity Extracellular Matrix (ECM) Alkaline Detergents Oscillating Pressure Conditions Histological Staining DNA Content Analysis Corrosion Casting
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