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Bioengineering

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JoVE Journal Bioengineering
3D Hydrogel Scaffolds for Articular Chondrocyte Culture and Cartilage Generation
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3D Hydrogel Scaffolds for Articular Chondrocyte Culture and Cartilage Generation

Article DOI: 10.3791/53085-v • 12:37 min • October 7th, 2015
October 7th, 2015 •
*1, *1,2, 1,3, 1
1Orthopaedic Surgery Department, Stanford University, 2Mechanical Engineering Department, Stanford University, 3Bioengineering Department, Stanford University
* These authors contributed equally

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résumé
Automatic Translation

Cartilage repair represents an unmet medical challenge and cell-based approaches to engineer human articular cartilage are a promising solution. Here, we describe three-dimensional (3D) biomimetic hydrogels as an ideal tool for the expansion and maturation of human articular chondrocytes.

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3D Hydrogel Scaffolds Articular Chondrocyte Culture Cartilage Generation Tissue Repair Cell Source Biomimetic Hydrogel Poly(ethylene Glycol) Chondroitin Sulfate Microenvironment In Vitro Culture Cell Delivery Cartilage Repair Gene Expression Immunofluorescence Staining Biochemical Assays Mechanical Testing
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