JoVE Journal
Immunology and Infection
Immunology and Infection
Ce contenu est en libre accès.
Chapitres
résumé
Here, a protocol is presented for creating an infectious bacterial artificial chromosome containing a full-length cDNA of the positive-strand genomic RNA of Japanese encephalitis virus. This protocol can be used to construct a functional cDNA of other positive-strand RNA viruses, making it a powerful genomic tool for studying virus biology.