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Fluorescence and Bioluminescence Imaging of Subcellular Ca2+ in Aged Hippocampal Neurons

Article DOI: 10.3791/53330-v • 13:24 min • December 1st, 2015
December 1st, 2015 •
1, 1, 2
1Instituto de Biologìa y Genética Molecular, Consejo Superior de Investigaciones Cientìficas, 2Departamento de Bioquìmica y Biologìa Molecular y Fisiologìa, Universidad de Valladolid

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résumé
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Intracellular Ca2+ remodeling in aging may contribute to excitotoxicity and neuron damage, processes mediated by Ca2+ overload. We aimed at investigating Ca2+ remodeling in the aging brain using fluorescence and bioluminescence imaging of cytosolic and mitochondrial Ca2+ in long-term cultures of rat hippocampal neurons, a model of neuronal aging.

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Fluorescence Bioluminescence Imaging Subcellular Ca2+ Aged Hippocampal Neurons Susceptibility Neuron Cell Death Neurodegeneration Ischemia Excitotoxicity Glutamate Receptors Ca2+ Influx Mitochondrial Ca2+ Overload Intracellular Ca2+ Homeostasis Remodeling Aging Live Cell Imaging Rat Hippocampal Neurons In Vivo Poli-D-lysine Coated Glass Coverslips Controlled Media Fura2 Cytosolic Ca2+ Concentration Digital Fluorescence Ratio Imaging Plasmids Low-affinity Aequorin GFP Mitochondria
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