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Immunology and Infection

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Intravital Imaging of Neutrophil Priming Using IL-1β Promoter-driven DsRed Reporter Mice

Article DOI: 10.3791/54070-v • 09:34 min • June 22nd, 2016
June 22nd, 2016 •
1,2, 1,3, 1
1Department of Medical Microbiology and Immunology, University of Toledo College of Medicine and Life Sciences, 2Department of Internal Medicine, Yale University School of Medicine, 3Department of Pathophysiology, Southern Medical University (China)

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This current protocol employs fluorescent reporters, in vivo labeling, and intravital imaging techniques to enable monitoring of the dynamic process of neutrophil priming in living animals.

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Intravital Imaging Neutrophil Priming IL-1β Promoter DsRed Reporter Mice Immunology Field Fluorescent Reporters Viral Labeling Intravital Imaging PIL1-DsRed Mice C57Black Six Mice Blood Collection Submandibular Vein Lancet Red Blood Cell Lysing Buffer Fetal Bovine Serum
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