A protocol establishing mesenchymal stem cells (MSCs) isolated from the synovial fluid and bone marrow of minipigs and non-invasively collected using syringe aspiration is presented. Cellular phenotyping was performed using flow cytometry after cell isolation and in vitro cultivation of bone marrow and synovial fluids derived from MSCs.
Lee, W., Park, J., Jang, S., Lee, S., Lee, H., Lee, J., Rho, G., Lee, S. Isolation and Cellular Phenotyping of Mesenchymal Stem Cells Derived from Synovial Fluid and Bone Marrow of Minipigs. J. Vis. Exp. (113), e54077, doi:10.3791/54077 (2016).