Application Fluorescence Resonance Energy Transfer (FRET) pour examiner effecteur translocation efficacité par<em> Coxiella burnetii</em> Pendant siRNA Silencing
10,668 Views
•
10:29 min
•
July 06, 2016
DOI:
Summary
Automatically generated
L'étude des interactions entre les bactéries pathogènes et leurs hôtes est un domaine important de la recherche biologique. Ici, nous décrivons les techniques nécessaires pour mesurer la translocation effecteur par Coxiella burnetii lors de l' inactivation de gène siRNA en utilisant un substrat BlaM.
Vidéos Connexes
08:51
Genome-wide RNAi Screening to Identify Host Factors That Modulate Oncolytic Virus Therapy
Vidéos Connexes
9042 Views
10:19
Screening and Identification of RNA Silencing Suppressors from Secreted Effectors of Plant Pathogens
Vidéos Connexes
6302 Views
N/A
Enforced Activation of Enhancer RNAs In Situ through the dCas9 Synergistic Activation Mediator System
Vidéos Connexes
2443 Views
06:26
Single Cell Analysis Of Transcriptionally Active Alleles By Single Molecule FISH
Vidéos Connexes
3130 Views
11:44
Generation and Multi-phenotypic High-content Screening of Coxiella burnetii Transposon Mutants
Vidéos Connexes
9952 Views
15:31
MISSION esiRNA for RNAi Screening in Mammalian Cells
Vidéos Connexes
16313 Views
13:40
Live Cell Calcium Imaging Combined with siRNA Mediated Gene Silencing Identifies Ca2+ Leak Channels in the ER Membrane and their Regulatory Mechanisms
Vidéos Connexes
18097 Views
10:58
Quantitative and Automated High-throughput Genome-wide RNAi Screens in C. elegans
Vidéos Connexes
17722 Views
10:44
In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing
Vidéos Connexes
1419 Views
10:29
Applying Fluorescence Resonance Energy Transfer (FRET) to Examine Effector Translocation Efficiency by Coxiella burnetii during siRNA Silencing
Vidéos Connexes
10.7K Views
Read Article
Cite this Article
Newton, P., Latomanski, E. A., Newton, H. J. Applying Fluorescence Resonance Energy Transfer (FRET) to Examine Effector Translocation Efficiency by Coxiella burnetii during siRNA Silencing. J. Vis. Exp. (113), e54210, doi:10.3791/54210 (2016).
Copy