Waiting
Traitement de la connexion…

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / Visualization of HIV-1 Gag Binding to Giant…
JoVE Journal
Immunology and Infection

Un abonnement à JoVE est nécessaire pour afficher ce contenu.

JoVE Journal Immunology and Infection
Visualization of HIV-1 Gag Binding to Giant Unilamellar Vesicle (GUV) Membranes
Isolation and Identification of Extravascular Immune Cells of the Heart
Journal (Immunology and Infection)
Isolation and Identification of Extravascular Immune Cells of the Heart
A Rapid Strategy for the Isolation of New Faustoviruses from Environmental Samples Using Vermamoeba vermiformis
Journal (Immunology and Infection)
A Rapid Strategy for the Isolation of New Faustoviruses from Environmental Samples Using Vermamoeba vermiformis
 

Visualization of HIV-1 Gag Binding to Giant Unilamellar Vesicle (GUV) Membranes

Article DOI: 10.3791/54293-v • 08:21 min • July 28th, 2016
July 28th, 2016 •
1, 2, 1
1Department of Microbiology and Immunology, University of Michigan Medical School, 2Department of Biophysics, University of Michigan

Chapitres

résumé
Automatic Translation

We illustrate here an in vitro membrane binding assay in which interactions between HIV-1 Gag and lipid membranes are visually analyzed using YFP-tagged Gag synthesized in a wheat germ-based in vitro translation system and GUVs prepared by an electroformation technique.

Tags

HIV-1 Gag Binding Giant Unilamellar Vesicle (GUV) Membranes Bio Assembly Lipid Chains Membrane Binding Virostructural Proteins Visual Detection Post Mixing Steps Lipid Stocks Heat Block Inorganic Solvents Glass Vials Parafilm Lipid Ratios Chloroform-resistant Device
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter