Surgical Approach for Middle Cerebral Artery Occlusion and Reperfusion Induced Stroke in Mice

Shantel A. Vital; Felicity N. E. Gavins

doi:10.3791/54302

Approche chirurgicale pour Occlusion de l'artère cérébrale moyenne et reperfusion induite par...

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Surgical Approach for Middle Cerebral Artery Occlusion and Reperfusion Induced Stroke in Mice

Approche chirurgicale pour Occlusion de l'artère cérébrale moyenne et reperfusion induite par des maladies chez les souris

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10:30 min

October 20, 2016

DOI: 10.3791/54302-v

Shantel A. Vital¹, Felicity N. E. Gavins¹

¹Department of Molecular & Cellular Physiology, Health Sciences Center Shreveport,Louisiana State University

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Overview

This article discusses the middle cerebral artery occlusion (MCAo) model, a widely used method for studying stroke in mice. The Longa method is highlighted for its ability to reproduce ischemia effectively, which is crucial for stroke research.

Key Study Components

Area of Science

Neuroscience
Stroke Research
Animal Models

Background

Ischemic strokes often result from MCA occlusion in humans.
Reliable models are essential for understanding stroke pathophysiology.
The Longa method is preferred due to its higher survival rates.
Increased leukocyte-endothelial interactions and infarct volume are observed with this technique.

Purpose of Study

To describe the MCAo model and its application in stroke research.
To identify therapeutic targets for stroke treatment.
To demonstrate the effectiveness of the Longa method in inducing ischemia.

Methods Used

Induction of anesthesia in male C57 black six mice.
Monitoring depth of anesthesia through foot pinch.
Application of sterile ocular ointment to prevent dryness.
Demonstration of the Longa method by laboratory manager Shantel Vital.

Main Results

The Longa method successfully reproduces ischemia at the MCA.
Higher survival rates compared to alternative techniques.
Increased leukocyte-endothelial interactions noted.
Significant infarct volume observed with this method.

Conclusions

The MCAo model is a reliable tool for stroke research.
The Longa method offers advantages in survival and reproducibility.
This model can aid in the discovery of new therapeutic strategies.

Frequently Asked Questions

What is the MCAo model?

The MCAo model is a widely used method for inducing stroke in mice, allowing researchers to study ischemia and potential treatments.

Why is the Longa method preferred?

The Longa method is preferred due to its higher survival rates and its ability to effectively reproduce ischemia.

What are the key advantages of using animal models in stroke research?

Animal models provide insights into the pathophysiology of stroke and allow for the testing of therapeutic interventions.

How does the Longa method affect leukocyte-endothelial interactions?

The Longa method increases leukocyte-endothelial cell interactions, which are important in the inflammatory response during stroke.

What is the significance of infarct volume in stroke studies?

Infarct volume is a critical measure of stroke severity and helps evaluate the effectiveness of potential treatments.

Who demonstrated the Longa method in this study?

Shantel Vital, the laboratory manager, demonstrated the Longa method in this study.

Afin de comprendre la physiopathologie de la course, il est important d'utiliser des modèles fiables. Ce document décrira l'un des modèles de course les plus fréquemment utilisés chez la souris, appelé le modèle milieu occlusion de l'artère cérébrale (MCAo) (également appelé le filament intraluminal ou d'un modèle de suture) avec reperfusion.

L’objectif global de la méthode Longa est de reproduire l’ischémie à la racine de l’artère cérébrale moyenne, ou ACM, car les accidents vasculaires cérébraux ischémiques chez l’homme se produisent le plus souvent en raison de l’occlusion de l’ACM. Cette méthode peut nous aider à répondre à des questions clés dans le domaine de l’AVC, telles que la recherche d’une cible thérapeutique appropriée pour le traitement de cette maladie. Le principal avantage de la méthode Longa par rapport aux techniques alternatives est le taux de survie.

Il y a également une augmentation des interactions leucocytaires-cellules endothéliales et du volume d’infarctus avec cette technique. Shantel Vital, la directrice de mon laboratoire, fera la démonstration de cette procédure. Après avoir induit l’anesthésie chez une souris mâle C57 black six selon le protocole de texte, surveillez la profondeur de l’anesthésie par un pincement du pied et appliquez une pommade oculaire stérile pour prévenir la sécheresse.

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