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Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production

Article DOI: 10.3791/54371-v • 10:10 min • September 20th, 2016
September 20th, 2016 •
1,2, 1,2, 1,2, 1,2, 1,2, 1,2,3, 1,2
1Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, 2NUS Synthetic Biology for Clinical and Technological Innovation (SynCTI), Life Sciences Institute, National University of Singapore, 3Food Science and Chemical Engineering, Singapore Institute of Technology

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résumé
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We herein report methods on the molecular genetic manipulation of the Yarrowia lipolytica Po1g strain for improved gene deletion efficiency. The resulting engineered Y. lipolytica strains have potential applications in biofuel and biochemical production.

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Genetic Engineering Unconventional Yeast Renewable Biofuel Biochemical Production DNA Transformation Gene Deletion Homologous Recombination Yarrowia Lipolytica PO1G Strain PCR-mediated Gene Disruption Selectable Markers Transformation Protocol Efficient Homologous Recombination Frequency LEU2 Expression Cassette LoxP Sites Cloning Process BamHI Restriction Site
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